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ENTRY TITLE

Neutralizing Monoclonal Antibody Generation to Androctonus species
IDEA BY: Brandon Russell LOCATION: USA CATEGORY: Science/Medical

ENTRY TITLE

Neutralizing Monoclonal Antibody Generation to Androctonus species
IDEA BY: Brandon Russell
LOCATION: USA
CATEGORY: Science/Medical

ENTRY DESCRIPTION

Generate a neutralizing murine monoclonal antibody to venom from Androctonus (fattail scorpion) species to utilize for developing a rapid diagnostic assay to test for envenomation in people as well as creating a humanized clone for antivenom treatment.

Androctonus species, located the Middle East, North and West Africa, are responsible for severe morbidity and mortality from envenomations in people every year. Although there is conventional antivenom available to treat patients that have been envenomated, the antivenom is of equine origin and thus increases the chances of an adverse reaction when administering in people. I propose to generate a monoclonal antibody of murine origin that can be used to develop a rapid diagnostic assay that can detect envenomation from Androctonus species in people. The ability to properly diagnose Androctonus envenomations is important because it is an affordable way to determine a proper treatment regime so that an individual does not unnecessarily receive antivenom avoiding both cost and potential adverse reactions to the patient. The benefit is not only to the patient but also the medical care provider to be able to conserve the antivenom which is becoming increasingly scarce. The murine derived neutralizing monoclonal antibody to Androctonus venom would have the potential to further be used to generate a humanized monoclonal antibody which can be produced in vitro and could replace traditional antivenom treatments. The availability of a humanized monoclonal antibody would be beneficial because mass quantities could be generated without the use of the scorpion or equine to manufacture the antivenom. The availability of a humanized monoclonal antibody would also be important because it would reduce the adverse reaction rate that is caused by traditional antivenom of equine origin since the humanized monoclonal antibody would be recognized and self.